Circular
permutation (CP) of protein is an evolutionary event resulting
in the fact that structural homologs may have different locations
of termini. It can be visualized as if the original termini
of a protein were linked and new ones created elsewhere. Since
circular permutants usually retain native structures and functions
with sometimes increased stabilities, activities or functional
diversities, CP has been applied as a protein engineering technique
in many fields. Considering that not every position in a protein
structure can be used to generate viable CP and the high cost
for creating CP by molecular cloning, CP site predictors, which
help avoid unnecessary trials-and-errors, will be beneficial
for the application of CP. Here we present CPred, the first
practical method for predicting viable CP sites.
References:
Lo WC, Wang LF, Liu YY, Dai T, Hwang JK, Lyu PC: CPred:
a web server for predicting viable circular permutations in
proteins.
Nucleic Acids Research 2012, doi:10.1093/nar/gks529. [Abstract]
[PDF]
Lo WC, Lee
CC, Lee CY, Lyu PC: CPDB: a database of circular permutation
in proteins.
Nucleic Acids Research 2009, 37:D328-332.
[Abstract]
[PDF]
Lo WC,
Lyu PC: CPSARST: an efficient circular permutation search
tool applied to the detection of novel protein
structural relationships.
Genome Biology 2008, 9:R11. [Abstract]
[PDF]
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